On-line enrichment and determination of aristolochic acid in medicinal plants using a MOF-based composite monolith as adsorbent

Miaomiao Zhang; Haiyan Liu; Yamei Han; Ligai Bai; Hongyuan Yan

doi:10.1016/j.jchromb.2020.122343

On-line enrichment and determination of aristolochic acid in medicinal plants using a MOF-based composite monolith as adsorbent

J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Nov 30:1159:122343. doi: 10.1016/j.jchromb.2020.122343. Epub 2020 Aug 28.

Authors

Miaomiao Zhang¹, Haiyan Liu², Yamei Han¹, Ligai Bai¹, Hongyuan Yan³

Affiliations

¹ Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
² Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China. Electronic address: lhy1610@126.com.
³ Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China. Electronic address: yanhongyuan@126.com.

PMID: 32905990
DOI: 10.1016/j.jchromb.2020.122343

Abstract

In this study, modified UiO-66-NH₂ and N-methylolacrylamide (NMA) were used as common monomers to prepare a metal organic framework (MOF)-based composite monolith through in-situ polymerization, which was used as a new adsorbent to purify and enrich aristolochic acid-I (AA-I) in medicinal plants. The MOF-based composite monolithic column was characterized by nitrogen adsorption-desorption isotherm, mercury intrusion porosimetry and scanning electron microscopy (SEM). The adsorption ability of MOF-based composite monolith for AA-I was compared with that of the polymer monolith without MOF added. The results proved that the addition of UiO-66-NH₂ can increase both the specific surface area and the permeability of the monolith. Moreover, the adsorption amount of AA-I on the monolith improved. This proposed on-line solid phase extraction (SPE) method showed good linear relationship in the range 0.044 ~ 400 μg/mL with r = 0.9994; the limit of detection (LOD) was 13.08 ng/mL and the limit of quantification (LOQ) was 44.00 ng/mL; the intra-day and inter-day accuracies were less than 0.97%; the inter-column accuracies was less than 6.11%; the recovery was in the range of 91.11%~106.48%. The method was found to be easy, accurate and convenient for on-line enrichment and purification of AA-I in medicinal plants.

Keywords: Aristolochic acids; Enrichment and determination; Medicinal plants; Metal organic frameworks; Solid-phase extraction.

MeSH terms

Aristolochic Acids / analysis*
Chromatography, High Pressure Liquid / methods
Limit of Detection
Linear Models
Metal-Organic Frameworks / chemistry*
Plants, Medicinal / chemistry*
Reproducibility of Results
Solid Phase Extraction / methods*

Substances

Aristolochic Acids
Metal-Organic Frameworks
aristolochic acid I