Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis

Xilong Kang; Chuang Meng; Zhiming Pan

doi:10.1007/978-1-0716-0791-6_17

Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis

Methods Mol Biol. 2021:2182:187-196. doi: 10.1007/978-1-0716-0791-6_17.

Authors

Xilong Kang^{1

2

3

4}, Chuang Meng^{1

2

3

4}, Zhiming Pan^{5

6

7

8}

Affiliations

¹ Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China.
² Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China.
³ Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA, Yangzhou University, Yangzhou, Jiangsu, China.
⁴ Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu, China.
⁵ Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China. zmpan@yzu.edu.cn.
⁶ Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China. zmpan@yzu.edu.cn.
⁷ Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA, Yangzhou University, Yangzhou, Jiangsu, China. zmpan@yzu.edu.cn.
⁸ Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu, China. zmpan@yzu.edu.cn.

PMID: 32894497
DOI: 10.1007/978-1-0716-0791-6_17

Abstract

Salmonella is recognized as a major human foodborne pathogen and threat to public health world widely. It is important to carry out epidemiological investigations to determine the primary sources of bacterial contamination. Pulsed-field gel electrophoresis (PFGE) is an important method of the molecular typing, and play an important role in tracking the sources of infection and epidemic control. The PFGE is currently considered as "gold standard" of molecular typing methods for bacterial foodborne pathogen. Here, we describe the PFGE protocol to type the Salmonella from pork.

Keywords: Molecular typing; Pulsed-field gel electrophoresis; Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
DNA, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field / methods*
Molecular Typing / methods*
Pork Meat / microbiology
Salmonella / genetics*

Substances

DNA, Bacterial