Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods

David Rodríguez-Lázaro; Marta Hernández

doi:10.1007/978-1-0716-0791-6_1

Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods

Methods Mol Biol. 2021:2182:1-6. doi: 10.1007/978-1-0716-0791-6_1.

Authors

David Rodríguez-Lázaro¹, Marta Hernández^{2

3}

Affiliations

¹ Microbiology Section, Faculty of Sciences, University of Burgos, Burgos, Spain. drlazaro@ubu.es.
² Microbiology Section, Faculty of Sciences, University of Burgos, Burgos, Spain.
³ Instituto Tecnológico Agrario (ITACyL), Junta de Castilla y León, Ctra. Burgos, Valladolid, Spain.

PMID: 32894481
DOI: 10.1007/978-1-0716-0791-6_1

Abstract

Molecular techniques such as real-time polymerase chain reaction (qPCR) have become a very effective alternative in food microbiology diagnostic for rapid and specific detection of foodborne pathogens such as Salmonella in foods and food-related environments. qPCR is a simple, sensitive, specific, and reproducible assay. Here, we describe the application of real-time PCR-based methods for a rapid (less than 24 h) detection of Salmonella in different types of foods fully compatible with the international standard for detection of Salmonella in food (ISO 6579-1:2017).

Keywords: Foods; Rapid detection; Real-time PCR; Salmonella spp..

MeSH terms

DNA, Bacterial / genetics
Food Microbiology / methods
Real-Time Polymerase Chain Reaction / methods*
Salmonella / genetics*
Sensitivity and Specificity

Substances

DNA, Bacterial