Visualization study on aniline-degrading bacteria AN-1 transport in the aquifer with the low-permeability lens

Xueming Qin; Yuduo Hua; He Sun; Jiayin Xie; Yongsheng Zhao

doi:10.1016/j.watres.2020.116329

Visualization study on aniline-degrading bacteria AN-1 transport in the aquifer with the low-permeability lens

Water Res. 2020 Nov 1:186:116329. doi: 10.1016/j.watres.2020.116329. Epub 2020 Aug 21.

Authors

Xueming Qin¹, Yuduo Hua², He Sun³, Jiayin Xie⁴, Yongsheng Zhao⁵

Affiliations

¹ National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, College of New Energy and Environment, Jilin University, Changchun 130021, China; Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: chingxm@163.com.
² National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, College of New Energy and Environment, Jilin University, Changchun 130021, China; Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: 3194096856@qq.com.
³ National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, College of New Energy and Environment, Jilin University, Changchun 130021, China; Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: 1726740591@qq.com.
⁴ National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, College of New Energy and Environment, Jilin University, Changchun 130021, China; Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: 592692413@qq.com.
⁵ National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, College of New Energy and Environment, Jilin University, Changchun 130021, China; Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: zhaoyongsheng@jlu.edu.cn.

PMID: 32889365
DOI: 10.1016/j.watres.2020.116329

Abstract

The geological conditions of the contaminated sites will affect the migration of microorganisms in the underground environment. In order to study the effect of low-permeability lens on bacterial transport, green fluorescent protein labeling combined with light transmission method was used to reveal the bacterial transport in the heterogeneous aquifer. The experiment has the advantages of real-time monitoring and no disturbance. The results showed that the bacteria gave priority to bypass the lens to flow away. The lens had a significant effect on hindering the bacterial transport due to adsorption and straining. The larger permeability coefficient ratio between the bulk media and the low-permeability lens was, the more obvious the obstruction was. AN-1 cannot enter the lens until the ratio decreased to the order of 10². With the increase of the flow velocity, the bacterial plume changed a lot. The higher flow velocity reduced the adsorption and retention of AN-1 to the media, resulting in some microorganisms remaining in the pores washed down. When the flow came to 2.0 m·d^-1, AN-1 cannot adhere to the media due to the excessive fluid shear stress.

Keywords: Bacterial transport; Groundwater; Heterogeneous; Light transmission; Low-permeability lens.

MeSH terms

Adsorption
Aniline Compounds
Bacteria
Groundwater*
Permeability
Water Pollutants, Chemical* / analysis

Substances

Aniline Compounds
Water Pollutants, Chemical