Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications

Kotoko Furuno; Jing Wang; Keiichiro Suzuki; Masaki Nakahata; Shinji Sakai

doi:10.1021/acsomega.0c03164

Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications

ACS Omega. 2020 Aug 13;5(33):21254-21259. doi: 10.1021/acsomega.0c03164. eCollection 2020 Aug 25.

Authors

Kotoko Furuno¹, Jing Wang¹, Keiichiro Suzuki^{1

2

3}, Masaki Nakahata¹, Shinji Sakai¹

Affiliations

¹ Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
² Institute for Advanced Co-Creation Studies, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
³ Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells.