Increased E. coli bio-adsorption resistance of microfiltration membranes, using a bio-inspired approach

Abstract

Cells have inherent anti-fouling properties. The mechanisms underpinning these natural properties inform the design of an anti-biosorption coating for a polyethersulfone microfiltration membrane, which includes polydopamine and chitosan layers. This tri-layered membrane is created using quick and easy synthesis method. Its ability to resist bio-adsorption and membrane extracellular polymeric substances (EPS) formation is investigated using the bacterium E. coli (ATCC 11775, 1.5 × 10⁷ CFU/mL). In addition, the proliferative bio-adsorption process is explored on the microfiltration membrane surface, using natural water under static and shaken conditions, while monitoring the bio-adsorption kinetics and EPS dynamic changes. The characterization results show that the modification by polydopamine and chitosan change the membrane surface morphology and increase its hydrophilicity. After 10 min dipping in 5 g/L chitosan solution, the pure water flux of the modified membrane is 5469 ± 30 L/(m²·h) (0.2 bar) and the contact angle decreases to 36.7 ± 1.0°, compared with 9889 ± 23 L/(m²·h) (0.2 bar) and 60.3 ± 1.5° for the unmodified polyethersulfone membrane, respectively. In proliferative bio-adsorption tests, the modified membrane is shown to decrease bio-adsorption by 0.4-2.3 orders of magnitude. However, no antimicrobial function is observed, probably due to the alkaline environment and insufficient functional amino groups. A series of linear and non-linear kinetic models is applied to fit the proliferative bio-adsorption process. The pseudo-second-order model is found to describe the proliferative bio-adsorption process best. Neither total organic carbon (TOC) nor protein is detected on the modified membrane surface. In contrast, on the unmodified PES membrane the ratios of protein/TOC (%), TOC/abundance ((μg/cm²)/CFU (log)) and protein/abundance ((μg/cm²)/CFU(log)) are 10%-16%, 0.17-0.28 and 0.02-0.04, respectively. No significant difference (p > 0.05) is found between static and shaken conditions. All these results point to improved anti-biosorption properties for water treatment applications, encouraging further studies on this membrane.

Keywords: Bio-adsorption; Characterization; Extracellular polymeric substances; Inspiration from nature; Membrane.