Integrated proteomic and transcriptomic analysis reveals that polymorphic shell colors vary with melanin synthesis in Bellamya purificata snail

Songqian Huang; Hanjun Jiang; Li Zhang; Qianhong Gu; Weimin Wang; Yanhong Wen; Fuguang Luo; Wu Jin; Xiaojuan Cao

doi:10.1016/j.jprot.2020.103950

Integrated proteomic and transcriptomic analysis reveals that polymorphic shell colors vary with melanin synthesis in Bellamya purificata snail

J Proteomics. 2021 Jan 6:230:103950. doi: 10.1016/j.jprot.2020.103950. Epub 2020 Aug 29.

Authors

Songqian Huang¹, Hanjun Jiang², Li Zhang³, Qianhong Gu⁴, Weimin Wang², Yanhong Wen⁵, Fuguang Luo⁵, Wu Jin⁶, Xiaojuan Cao⁷

Affiliations

¹ College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, the University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
² College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
³ College of Marxism, Shanghai University of Finance and Economics, Shanghai 200433, China.
⁴ The State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
⁵ Liuzhou Aquaculture Technology Extending Station, Liuzhou, China.
⁶ Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China. Electronic address: jinw@ffrc.cn.
⁷ College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: caoxiaojuan@mail.hzau.edu.cn.

PMID: 32871245
DOI: 10.1016/j.jprot.2020.103950

Abstract

The snail Bellamya purificata is an ecologically and economically important freshwater gastropod species. However, limited genomic resources are available for this snail. In this study, the transcriptome of mantle tissues and proteome of shells of B. purificata with two shell colors (namely light-cyan line (LC) and light-purple line (LP)) were deeply sequenced and characterized. A total of 5.72 million contigs were assembled into 157,015 unigenes, 21,455 (13.66%) of these unigenes were significantly matched to NR, Swiss-Prot, KOG, GO and KEGG database. 1807 differentially expressed genes (DEGs) were identified between the two different shell color lines. These DEGs were significantly enriched in five KEGG pathways including tyrosine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and histidine metabolism, which suggested that the shell color polymorphism in B. purificata was a result of melanin synthesis variation. A total of 1521 proteins were identified in B. purificata here as well. The differentially expressed protein analysis showed that the tyrosinase content in LP was significantly decreased in comparison to LC, which agreed with the transcriptome analysis results. This study provides valuable genomic resources of B. purificata and improves our understanding of molecular mechanisms of biomineralization and shell color polymorphism in snail.

Keywords: Bellamya purificata; Proteome; Shell colors; Transcriptome; Tyrosinase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Color
Gene Expression Profiling
Melanins* / genetics
Proteomics
Snails / genetics
Transcriptome*

Substances

Melanins