Full-field optical coherence tomography for the diagnosis of giant cell arteritis

PLoS One. 2020 Aug 31;15(8):e0234165. doi: 10.1371/journal.pone.0234165. eCollection 2020.

Abstract

Histopathological examination of temporal artery biopsy (TAB) remains the gold standard for the diagnosis of giant cell arteritis (GCA) but is associated with essential limitations that emphasize the need for an upgraded pathological process. This study pioneered the use of full-field optical coherence tomography (FF-OCT) for rapid and automated on-site pathological diagnosis of GCA. Sixteen TABs (12 negative and 4 positive for GCA) were selected according to major histopathological criteria of GCA following hematoxylin-eosin-saffron-staining for subsequent acquisition with FF-OCT to compare structural modifications of the artery cell wall and thickness of each tunica. Gabor filtering of FF-OCT images was then used to compute TAB orientation maps and validate a potential automated analysis of TAB sections. FF-OCT allowed both qualitative and quantitative visualization of the main structures of the temporal artery wall, from the internal elastic lamina to the vasa vasorum and red blood cells, unveiling a significant correlation with conventional histology. FF-OCT imaging of GCA TABs revealed destruction of the media with distinct remodeling of the whole arterial wall into a denser reticular fibrous neo-intima, which is distinctive of GCA pathogenesis and accessible through automated Gabor filtering. Rapid on-site FF-OCT TAB acquisition makes it possible to identify some characteristic pathological lesions of GCA within a few minutes, paving the way for potential machine intelligence-based or even non-invasive diagnosis of GCA.

Trial registration: ClinicalTrials.gov NCT02158208 NCT02857192.

Publication types

  • Clinical Trial
  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Cohort Studies
  • Female
  • Giant Cell Arteritis / diagnosis
  • Giant Cell Arteritis / diagnostic imaging*
  • Giant Cell Arteritis / pathology
  • Humans
  • Male
  • Middle Aged
  • Prospective Studies
  • Temporal Arteries / diagnostic imaging
  • Temporal Arteries / pathology
  • Tomography, Optical Coherence / methods*
  • Tunica Intima / diagnostic imaging
  • Tunica Intima / pathology
  • Tunica Media / diagnostic imaging
  • Tunica Media / pathology

Associated data

  • ClinicalTrials.gov/NCT02158208
  • ClinicalTrials.gov/NCT02857192

Grants and funding

This work was supported by grants from the 'Fondation ARTHRITIS (2017-2018)' and the 'Groupement Interrégional de Recherche Clinique et d’Innovation Est (GIRCI) - Appel à Projet Jeunes Chercheurs' 2013 and 2014 (Clinicaltrials.gov NCT02158208 and NCT02857192). The funder did not provide any salary for authors, had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section. LLTech SAS did not play any role as a funding organization for this study.