Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity

Evelina L Zdorovenko; Alexandra A Kadykova; Alexander S Shashkov; Liudmyla D Varbanets; Tetiana V Bulyhina; Yuriy A Knirel

doi:10.1016/j.carres.2020.108132

Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity

Carbohydr Res. 2020 Oct:496:108132. doi: 10.1016/j.carres.2020.108132. Epub 2020 Aug 15.

Authors

Evelina L Zdorovenko¹, Alexandra A Kadykova², Alexander S Shashkov², Liudmyla D Varbanets³, Tetiana V Bulyhina³, Yuriy A Knirel²

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia. Electronic address: zdorovenkoe@mail.ru.
² N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia.
³ D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143, Kiev, Ukraine.

PMID: 32861900
DOI: 10.1016/j.carres.2020.108132

Abstract

Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods: The LPS of Р. agglomerans 7460 showed low level of toxicity and pyrogenicity to compare with LPS of E. coli O55:B5 and pyrogenal, respectively. The ability of the modified (succinylated) LPS, which have lost its toxicity, to block the toxic effects of native LPS has been shown.

Keywords: Anti-LPS strategy; Lipid A; O-polysaccharide structure; Pantoea agglomerans; Pyrogenicity; Toxicity.

MeSH terms

Carbohydrate Conformation
Models, Molecular
O Antigens / chemistry*
O Antigens / isolation & purification
O Antigens / toxicity
Pantoea / chemistry*
Phenol / chemistry
Phosphorylation
Water / chemistry

Substances

O Antigens
Water
Phenol