Long-term effects of maternal cannabis vapor exposure on emotional reactivity, social behavior, and behavioral flexibility in offspring

Halle V Weimar; Hayden R Wright; Collin R Warrick; Amanda M Brown; Janelle M Lugo; Timothy G Freels; Ryan J McLaughlin

doi:10.1016/j.neuropharm.2020.108288

Long-term effects of maternal cannabis vapor exposure on emotional reactivity, social behavior, and behavioral flexibility in offspring

Neuropharmacology. 2020 Nov 15:179:108288. doi: 10.1016/j.neuropharm.2020.108288. Epub 2020 Aug 26.

Authors

Halle V Weimar¹, Hayden R Wright¹, Collin R Warrick², Amanda M Brown¹, Janelle M Lugo¹, Timothy G Freels¹, Ryan J McLaughlin³

Affiliations

¹ Departments of Integrative Physiology and Neuroscience, Washington State University, WA, USA.
² Departments of Integrative Physiology and Neuroscience, Washington State University, WA, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, MD, USA.
³ Departments of Integrative Physiology and Neuroscience, Washington State University, WA, USA; Department of Psychology, Washington State University, WA, USA. Electronic address: ryan.mclaughlin@wsu.edu.

PMID: 32860776
DOI: 10.1016/j.neuropharm.2020.108288

Abstract

The use of cannabis during pregnancy is a growing public health concern. As more countries implement legislation permitting recreational cannabis use, there is an urgent need to better understand its impact on fetal neurodevelopment and its long-term effects in exposed offspring. Studies examining effects of prenatal cannabis exposure typically employ injections of synthetic cannabinoids or isolated cannabis constituents that may not accurately model cannabis use in human populations. To address this limitation, we developed a novel e-cigarette technology-based system to deliver vaporized cannabis extracts to pregnant Long Evans rats. We used this model to determine effects of prenatal cannabis exposure on emotional, social, and cognitive endpoints of male and female offspring during early development and into adulthood. Dams were exposed to cannabis vapor (CAN_THC: 400 mg/ml), vehicle vapor (VEH), or no vapor (AIR) twice daily during mating and gestation. Offspring exposed to CAN_THC and VEH showed reduced weight gain relative to AIR offspring prior to weaning. CAN_THC offspring made more isolation-induced ultrasonic vocalizations (USVs) on postnatal day 6 (P6) relative to VEH-exposed offspring, which is indicative of increased emotional reactivity. Male CAN_THC offspring engaged in fewer social investigation behaviors than VEH-exposed male offspring during a social play test on P26. In adulthood, CAN_THC-exposed offspring spent less time exploring the open arms of the elevated plus maze and exhibited dose-dependent deficits in behavioral flexibility in an attentional set-shifting task relative to AIR controls. These data collectively indicate that prenatal cannabis exposure may cause enduring effects on the behavioral profile of offspring.

Keywords: Anxiety; Behavioral flexibility; Emotional reactivity; Prenatal cannabis exposure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cannabis / adverse effects*
Emotions / drug effects*
Emotions / physiology
Female
Inhalation Exposure / adverse effects*
Male
Maternal Exposure / adverse effects*
Pregnancy
Prenatal Exposure Delayed Effects / chemically induced*
Prenatal Exposure Delayed Effects / psychology
Rats
Rats, Long-Evans
Social Behavior*
Vocalization, Animal / drug effects
Vocalization, Animal / physiology