Germ Cell Tumor Cell Culture Techniques

John T Lafin; James F Amatruda; Aditya Bagrodia

doi:10.1007/978-1-0716-0860-9_5

Germ Cell Tumor Cell Culture Techniques

Methods Mol Biol. 2021:2195:65-76. doi: 10.1007/978-1-0716-0860-9_5.

Authors

John T Lafin¹, James F Amatruda², Aditya Bagrodia³

Affiliations

¹ Department of Urology, University of Texas Medical Center, Dallas, TX, USA.
² Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
³ Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA. aditya.bagrodia@utsouthwestern.edu.

PMID: 32852757
DOI: 10.1007/978-1-0716-0860-9_5

Abstract

Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture. Additionally, methods for measuring cell viability and proliferation validated on these lines are provided.

Keywords: Cell culture; Embryonal carcinoma; Germ cell tumor; Seminoma; Yolk sac tumor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques*
Cell Line, Tumor
Cell Survival
Cryopreservation / methods
Humans
Neoplasms, Germ Cell and Embryonal / diagnosis
Neoplasms, Germ Cell and Embryonal / pathology*
Primary Cell Culture*