Antagonistic Interaction between Auxin and SA Signaling Pathways Regulates Bacterial Infection through Lateral Root in Arabidopsis

Xiangpei Kong; Chunlei Zhang; Huihui Zheng; Min Sun; Feng Zhang; Mengyue Zhang; Fuhao Cui; Dongping Lv; Lijing Liu; Siyi Guo; Youming Zhang; Xianzheng Yuan; Shan Zhao; Huiyu Tian; Zhaojun Ding

doi:10.1016/j.celrep.2020.108060

Antagonistic Interaction between Auxin and SA Signaling Pathways Regulates Bacterial Infection through Lateral Root in Arabidopsis

Cell Rep. 2020 Aug 25;32(8):108060. doi: 10.1016/j.celrep.2020.108060.

Authors

Xiangpei Kong¹, Chunlei Zhang², Huihui Zheng², Min Sun², Feng Zhang², Mengyue Zhang², Fuhao Cui³, Dongping Lv⁴, Lijing Liu², Siyi Guo⁵, Youming Zhang⁶, Xianzheng Yuan⁷, Shan Zhao⁷, Huiyu Tian², Zhaojun Ding⁸

Affiliations

¹ The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, Shandong, China. Electronic address: kongxiangpei@sdu.edu.cn.
² The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, Shandong, China.
³ Department of Plant Pathology and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, China.
⁴ State Key Laboratory of Plant Genomics, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050021, China.
⁵ Key Laboratory of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, China.
⁶ State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.
⁷ Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, Shandong, China.
⁸ The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, Shandong, China; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China. Electronic address: dingzhaojun@sdu.edu.cn.

PMID: 32846118
DOI: 10.1016/j.celrep.2020.108060

Abstract

Pathogen entry into host tissues is a critical and first step in infections. In plants, the lateral roots (LRs) are a potential entry and colonization site for pathogens. Here, using a GFP-labeled pathogenic bacterium Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), we observe that virulent Pto DC3000 invades plants through emerged LRs in Arabidopsis. Pto DC3000 strongly induced LR formation, a process that was dependent on the AUXIN RESPONSE FACTOR7 (ARF7)/ARF19-LATERAL ORGAN BOUNDARIES-DOMAIN (LBD) regulatory module. We show that salicylic acid (SA) represses LR formation, and several mutants defective in SA signaling are also involved in Pto DC3000-induced LR development. Significantly, ARF7, a well-documented positive regulator of LR development, directly represses the transcription of PR1 and PR2 to promote LR development. This study indicates that ARF7-mediated auxin signaling antagonizes with SA signaling to control bacterial infection through the regulation of LR development.

Keywords: ARF7; PR1; PR2; Pto DC3000; SA; auxin; bacterial infection; lateral root.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis
Bacterial Infections / microbiology*
Indoleacetic Acids / metabolism*
Plant Roots / chemistry*
Signal Transduction

Substances

Indoleacetic Acids