The assessment of parasympathetic nervous activity and psychophysiological responses infers the stress imposed by different resistance training systems. Therefore, we compare the effects of different sets configurations, with similar volume (~60 repetitions), on heart rate variability indices and internal training load. Twenty-nine resistance-trained adults completed the following conditions: traditional without and with muscle failure, inter-repetition rest, and rest-pause in the parallel squat. The heart rate variability indices (time-domain) were measured before and 30 min after each condition. The internal training load was obtained through the session-rating of perceived exertion method. Except for inter-repetition rest, all conditions reduced the heart rate variability indices after the session (P<0.05), and the rest-pause triggered the higher reductions (≤-46.7%). The internal training load was higher in the rest-pause (≤68.9%). Our results suggest that rest-pause configuration leads to more considerable disruption of the parasympathetic nervous activity and higher internal training load in trained adults. In contrast, inter-repetition rest allows lower autonomic and psychophysiological stress.
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