Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Muhammad A Asif; Melissa Garcia; Joanne Tilbrook; Chris Brien; Kate Dowling; Bettina Berger; Rhiannon K Schilling; Laura Short; Christine Trittermann; Matthew Gilliham; Delphine Fleury; Stuart J Roy; Allison S Pearson

doi:10.1071/FP20167

Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Funct Plant Biol. 2021 Jan;48(2):131-140. doi: 10.1071/FP20167.

Authors

Affiliations

¹ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
² Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and ARC Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia.
³ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and Australian Plant Phenomics Facility, The Plant Accelerator, The University of Adelaide, SA 5064, Australia; and School of Information Technology and Mathematical Sciences, The University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia.
⁴ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and Australian Plant Phenomics Facility, The Plant Accelerator, The University of Adelaide, SA 5064, Australia.
⁵ School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and Australian Plant Phenomics Facility, The Plant Accelerator, The University of Adelaide, SA 5064, Australia.
⁶ School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and ARC Centre of Excellence in Plant Energy Biology, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
⁷ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and ARC Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia; and Innolea, 6 chemin de Panedautes, 31700, Mondonville, France.
⁸ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and ARC Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia; and Corresponding author. Email: stuart.roy@adelaide.edu.au.
⁹ Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia; and School of Agriculture, Food and Wine & Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia; and ARC Centre of Excellence in Plant Energy Biology, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.

PMID: 32835651
DOI: 10.1071/FP20167

Abstract

Bread wheat (Triticum aestivum L.) is one of the most important food crops, however it is only moderately tolerant to salinity stress. To improve wheat yield under saline conditions, breeding for improved salinity tolerance of wheat is needed. We have identified nine quantitative trail loci (QTL) for different salt tolerance sub-traits in a recombinant inbred line (RIL) population, derived from the bi-parental cross of Excalibur × Kukri. This population was screened for salinity tolerance subtraits using a combination of both destructive and non-destructive phenotyping. Genotyping by sequencing (GBS) was used to construct a high-density genetic linkage map, consisting of 3236 markers, and utilised for mapping QTL. Of the nine mapped QTL, six were detected under salt stress, including QTL for maintenance of shoot growth under salinity (QG(1-5).asl-5A, QG(1-5).asl-7B) sodium accumulation (QNa.asl-2A), chloride accumulation (QCl.asl-2A, QCl.asl-3A) and potassium:sodium ratio (QK:Na.asl-2DS2). Potential candidate genes within these QTL intervals were shortlisted using bioinformatics tools. These findings are expected to facilitate the breeding of new salt tolerant wheat cultivars.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Genetic Linkage
Genotype
Plant Breeding
Quantitative Trait Loci
Salt Tolerance* / genetics
Triticum* / genetics