Determination of Sulfonamides in Milk by Cloud Point-Salting Out Extraction and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

Anal Sci. 2020 Dec 10;36(12):1555-1559. doi: 10.2116/analsci.20N013. Epub 2020 Aug 21.

Abstract

A method involving cloud point-salting out extraction (CPSOE) coupled with UHPLC-MS/MS was developed for the determination of eleven sulfonamides in milk. In this study, the type and concentration of the surfactant, de-emulsification condition, pH value, volume of n-butanol, equilibration temperature and time were optimized. For this developed method, the linear range of SAs was from 0.05 to 50 μg L-1, and the correlation coefficients were higher than 0.997. The average recoveries for SAs were from 61.32 to 91.67%, and the LOQs were less than 0.06 μg kg-1.

Keywords: Cloud point-salting out extraction; UPLC-MS/MS; milk; sulfonamides.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid*
  • Food Analysis / methods*
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Milk / chemistry*
  • Salts / chemistry*
  • Sulfonamides / analysis*
  • Sulfonamides / isolation & purification*
  • Surface-Active Agents / chemistry
  • Tandem Mass Spectrometry*

Substances

  • Salts
  • Sulfonamides
  • Surface-Active Agents