On-line dissolution analysis of multiple drugs encapsulated in electrospun nanofibers

Abstract

Electrospun nanofiber is a very attractive material which can be used as the support to form multiple-drug dosage. Understanding the dissolution process of different active drug ingredients released from electrospun fibers is of great importance to control and evaluate the quality of medicated nanofibers. Here we present the first study of on-line automatic analysis of dissolution of multiple drugs in electrospun fiber mats. Single-needle electrospinning technology is utilized to combine polymers, hydrophilic polyvinylpyrrolidone (PVP) and hydrophobic polycaprolactone (PCL) as carrier to load three poorly water-soluble non-steroidal anti-inflammatory drugs (paracetamol, nimesulide, and ibuprofen). The loading of the drugs in PVP/PCL electrospun fibers are characterized by various techniques, including scanning electron microscopy, X-ray diffraction, Fourier infrared spectroscopy and differential scanning calorimetry. The in vitro dissolution is investigated by our home-made portable analyzer, which can simultaneously on-line determine multiple drugs released from the nanofibers by a single step. The analysis shows a wide linear detection range of the drugs with limit-of-detection (LOD) down to μg/mL-level. The dissolution profiles of three ingredients in nanofibers can be monitored every thirty seconds from the beginning to the end in the entire dissolution process from only one HSCE run. The kinetic information of the dissolution, including the dissolution curve, characteristic dissolution time and dissolution efficiency, is obtained and evaluated for different dissolution media, drug loading content and the ratio of PVP/PCL. Our study provides a promising method for rapid and accurate dissolution testing of nanofiber-based drugs, and would extend the applications of separation techniques in pharmaceutical analysis.

Keywords: Dissolution profile; Drug release; Electrospun nanofibers; Multiple drugs.