Influence of the particle size of gadolinium-loaded chitosan nanoparticles on their tumor-killing effect in neutron capture therapy in vitro

Tooru Andoh; Yugo Nakatani; Minoru Suzuki; Yoshinori Sakurai; Takuya Fujimoto; Hideki Ichikawa

doi:10.1016/j.apradiso.2020.109270

Influence of the particle size of gadolinium-loaded chitosan nanoparticles on their tumor-killing effect in neutron capture therapy in vitro

Appl Radiat Isot. 2020 Oct:164:109270. doi: 10.1016/j.apradiso.2020.109270. Epub 2020 Jul 5.

Authors

Tooru Andoh¹, Yugo Nakatani², Minoru Suzuki³, Yoshinori Sakurai⁴, Takuya Fujimoto⁵, Hideki Ichikawa⁶

Affiliations

¹ Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, 650-8586, Japan. Electronic address: t.andoh@pharm.kobegakuin.ac.jp.
² Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, 650-8586, Japan.
³ Particle Radiation Oncology, Institute for Integrated Radiation and Nuclear Science, Kyoto University, Sennan-gun, 590-0494, Japan.
⁴ Particle Radiation Medical Physics, Institute for Integrated Radiation and Nuclear Science, Kyoto University, Sennan-gun, 590-0494, Japan.
⁵ Department of Orthopaedic Surgery, Hyogo Cancer Center, Akashi, 673-8558, Japan.
⁶ Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, 650-8586, Japan. Electronic address: ichikawa@pharm.kobegakuin.ac.jp.

PMID: 32819508
DOI: 10.1016/j.apradiso.2020.109270

Abstract

Neutron capture therapy using ¹⁵⁷Gd (Gd-NCT) is currently under development as a cancer radiotherapy. Melanoma cells were treated with gadolinium-loaded chitosan nanoparticles (Gd-nanoCPs) for Gd-NCT. Smaller Gd-nanoCPs had higher Gd content and better cellular association of Gd and thereby made the tumor-killing effect more efficient in comparison to larger Gd-nanoCPs. This indicates that Gd-nanoCP size reduction is an efficient method for improving the cellular affinity of Gd-nanoCPs and for enhancing the tumor-killing effect of Gd-NCT.

Keywords: Chitosan; Gadolinium; Nanoparticle; Neutron capture therapy.

MeSH terms

Animals
Cell Proliferation
Chitosan / chemistry*
Gadolinium / chemistry*
Melanoma, Experimental / pathology
Melanoma, Experimental / radiotherapy*
Mice
Molecular Weight
Nanoparticles / chemistry*
Neutron Capture Therapy / methods*
Particle Size

Substances

Chitosan
Gadolinium