Abstract
The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells.
Keywords:
Cervical Cancer; HPV16 E6/E7; NUDT21; miR-4454; α/β-hydrolase domain-containing 2.
© 2020 The Author(s).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / genetics
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Cell Line, Tumor
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Cell Movement / genetics
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Cell Proliferation / genetics
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Cleavage And Polyadenylation Specificity Factor / genetics
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Cleavage And Polyadenylation Specificity Factor / metabolism
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Hydrolases / genetics
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Hydrolases / metabolism
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / metabolism*
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Papillomavirus E7 Proteins / genetics
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Papillomavirus E7 Proteins / metabolism*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Up-Regulation
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Uterine Cervical Neoplasms / genetics
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Uterine Cervical Neoplasms / pathology*
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Uterine Cervical Neoplasms / virology*
Substances
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Cleavage And Polyadenylation Specificity Factor
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E6 protein, Human papillomavirus type 16
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MIRN4454 microRNA, human
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MicroRNAs
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Nudt21 protein, human
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Oncogene Proteins, Viral
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Papillomavirus E7 Proteins
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Repressor Proteins
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oncogene protein E7, Human papillomavirus type 16
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Hydrolases
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ABHD2 protein, human