A Single-Transaminase-Catalyzed Biocatalytic Cascade for Efficient Asymmetric Synthesis of l-Phosphinothricin

Feng Cheng; Ju-Mou Li; Shi-Peng Zhou; Qi Liu; Li-Qun Jin; Ya-Ping Xue; Yu-Guo Zheng

doi:10.1002/cbic.202000488

A Single-Transaminase-Catalyzed Biocatalytic Cascade for Efficient Asymmetric Synthesis of l-Phosphinothricin

Chembiochem. 2021 Jan 15;22(2):345-348. doi: 10.1002/cbic.202000488. Epub 2020 Sep 28.

Authors

Feng Cheng¹, Ju-Mou Li¹, Shi-Peng Zhou¹, Qi Liu¹, Li-Qun Jin¹, Ya-Ping Xue¹, Yu-Guo Zheng¹

Affiliation

¹ The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.

PMID: 32815302
DOI: 10.1002/cbic.202000488

Abstract

A single-transaminase-catalyzed biocatalytic cascade was developed by employing the desired biocatalyst, ATA-117-Rd11, that showed high activity toward 2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid (PPO) and α-ketoglutarate, and low activity against pyruvate. The cascade successfully promotes a highly asymmetric amination reaction for the synthesis of l-phosphinothricin (l-PPT) with high conversion (>95 %) and>99 % ee. In a scale-up experiment, using 10 kg pre-frozen E. coli cells harboring ATA-117-Rd11 as catalyst, 80 kg PPO was converted to ≈70 kg l-PPT after 24 hours with a high ee value (>99 %).

Keywords: L-phosphinothricin; biocatalysis; enzyme cascades; transaminase; virtual screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aminobutyrates / chemistry
Aminobutyrates / metabolism*
Biocatalysis
Molecular Structure
Transaminases / metabolism*

Substances

Aminobutyrates
phosphinothricin
Transaminases