Ribosomes clear the way for siRNA targeting

Nat Struct Mol Biol. 2020 Sep;27(9):775-777. doi: 10.1038/s41594-020-0495-4.

Abstract

Visualizing siRNA targeting of single mRNAs in living cells reveals that passing ribosomes temporarily unfold the mRNA, exposing it to siRNA recognition. This effect depends on the slow reorganization of many weak suboptimal interactions within the mRNA.

Publication types

  • News

MeSH terms

  • Animals
  • Binding Sites
  • Humans
  • Protein Biosynthesis
  • RNA Folding
  • RNA Interference
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism
  • Ribosomes / genetics*
  • Ribosomes / metabolism

Substances

  • RNA, Messenger
  • RNA, Small Interfering