Abstract
Visualizing siRNA targeting of single mRNAs in living cells reveals that passing ribosomes temporarily unfold the mRNA, exposing it to siRNA recognition. This effect depends on the slow reorganization of many weak suboptimal interactions within the mRNA.
MeSH terms
-
Animals
-
Binding Sites
-
Humans
-
Protein Biosynthesis
-
RNA Folding
-
RNA Interference
-
RNA, Messenger / genetics*
-
RNA, Messenger / metabolism
-
RNA, Small Interfering / genetics*
-
RNA, Small Interfering / metabolism
-
Ribosomes / genetics*
-
Ribosomes / metabolism
Substances
-
RNA, Messenger
-
RNA, Small Interfering