HPLC/ESI-MS identification of diastereomers of Impurity E - Degradation products of cefaclor

Klaudia Chuchracka; Rafał Frański; Magdalena Frańska

doi:10.1016/j.jpba.2020.113533

HPLC/ESI-MS identification of diastereomers of Impurity E - Degradation products of cefaclor

J Pharm Biomed Anal. 2020 Oct 25:190:113533. doi: 10.1016/j.jpba.2020.113533. Epub 2020 Aug 12.

Authors

Klaudia Chuchracka¹, Rafał Frański², Magdalena Frańska³

Affiliations

¹ Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.
² Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland. Electronic address: franski@amu.edu.pl.
³ Institute of Chemistry and Technical Electrochemistry, Poznań University of Technology, Berdychowo 4, 60-965 Poznań, Poland.

PMID: 32814261
DOI: 10.1016/j.jpba.2020.113533

Abstract

A seven years old sample of cefaclor stored in room conditions, as a solid, has been analyzed by using high performence liquid chromatography/electrospray ionization-mass spectrometry. As a result of the sample degradation process, two diastereomers of Impurity E have been found to have formed. It is reasonable to assume that the diastereomers are formed due to the isomerization of C6 carbon atom. They have almost identical fragmentation patterns in both positive and negative ion mode. On the other hand, the diastereomers have different efficiencies of formation of dimer ions, under ESI conditions, especially in negative ion mode.

Keywords: Cefaclor; Degradation; Diastereomer; Electrospray ionization; Impurity E; LC/MS.

MeSH terms

Cefaclor*
Chromatography, High Pressure Liquid
Chromatography, Liquid
Ions
Spectrometry, Mass, Electrospray Ionization*

Substances

Ions
Cefaclor