Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor

Erika Cosset; Tieng Vannary; Frédérique Sloan-Béna; Stefania Gimelli; Eric Gerstel; Karl-Heinz Krause; Antoine Marteyn

doi:10.1016/j.scr.2020.101928

Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor

Stem Cell Res. 2020 Oct:48:101928. doi: 10.1016/j.scr.2020.101928. Epub 2020 Aug 3.

Authors

Erika Cosset¹, Tieng Vannary², Frédérique Sloan-Béna³, Stefania Gimelli³, Eric Gerstel⁴, Karl-Heinz Krause⁵, Antoine Marteyn⁶

Affiliations

¹ Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
² Vanarix SA, Lausanne, Switzerland.
³ Service of Genetic Medicine, Geneva University Hospitals, Geneva, Switzerland.
⁴ Hirslanden Clinique La Colline, Geneva, Switzerland; Department of Internal Medicine, Department of Primary Care Medicine, Clinical Epidemiology Division, Department of Community Health and Medicine, Geneva University Hospital, Geneva, Switzerland.
⁵ Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
⁶ Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Geneva, Switzerland. Electronic address: antoine.marteyn@unige.ch.

PMID: 32805538
DOI: 10.1016/j.scr.2020.101928

Abstract

Dermal fibroblasts isolated from an apparently healthy 50-year-old man were successfully transformed into induced pluripotent stem cells (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The generated iPSC line has been expanded under feeder-free conditions and displayed all hallmarks of a standard pluripotent stem cell line such as a normal karyotype, expression of pluripotent factors and differentiation capacity into the three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Male
Middle Aged