Circular RNA DGKB Promotes the Progression of Neuroblastoma by Targeting miR-873/GLI1 Axis

Jiale Yang; Leitao Yu; Jinlong Yan; Yu Xiao; Weiming Li; Juhua Xiao; Jun Lei; Deng Xiang; Shouhua Zhang; Xin Yu

doi:10.3389/fonc.2020.01104

Circular RNA DGKB Promotes the Progression of Neuroblastoma by Targeting miR-873/GLI1 Axis

Front Oncol. 2020 Jul 23:10:1104. doi: 10.3389/fonc.2020.01104. eCollection 2020.

Authors

Jiale Yang¹, Leitao Yu¹, Jinlong Yan¹, Yu Xiao², Weiming Li², Juhua Xiao³, Jun Lei², Deng Xiang², Shouhua Zhang², Xin Yu¹

Affiliations

¹ Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, China.
² Department of General Surgery, Jiangxi Provincial Children's Hospital, Nanchang, China.
³ Department of Ultrasound, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, China.

Abstract

Accumulated evidences suggested that circular RNAs (circRNA) played critical roles in tumorigenesis and progression. To our knowledge, no study reported the function of circular RNA DGKB (circDGKB, circRNA ID: hsa_circ_0133622) on progression of neuroblastoma (NB). Here, we showed that circDGKB was upregulated in NB tissues compared to the normal dorsal root ganglia. Moreover, the expression level of circDGKB was negatively correlated with the survival rate of NB patients. Mechanically, overexpression of circDGKB promoted the proliferation, migration, invasion, and tumorigenesis of NB cells and reduced cell apoptosis, and vice versa. In addition, qRT-PCR and/or Western blot results showed that circDGKB overexpression inhibited the expression level of miR-873 and enhanced GLI1 expression. Moreover, miR-873 functioned an opposite role to circDGKB and significantly weakened circDGKB role in promoting NB progression. Furthermore, GLI1 upregulation also rescued the miR-873 role in inhibiting NB progression. In conclusion, our work proved that circDGKB promoted NB progression via targeting miR-873/GLI1 axis in vitro and in vivo. Our study provided a new target for NB treatment and indicated that circDGKB could act as a novel diagnostic marker for NB.

Keywords: GLI1; circDGKB; diagnostic marker; miR-873; neuroblastoma.