Influence of dl-3-N-butylphthalide on infarction size in rats with acute myocardial infarction

Yanbo Wang; Guozhen Hao; Yunfa Jiang; Wei Zhi; Qing Wang; Xinshun Gu

Influence of dl-3-N-butylphthalide on infarction size in rats with acute myocardial infarction

Int J Clin Exp Pathol. 2020 Jul 1;13(7):1707-1711. eCollection 2020.

Authors

Yanbo Wang¹, Guozhen Hao¹, Yunfa Jiang¹, Wei Zhi¹, Qing Wang¹, Xinshun Gu¹

Affiliation

¹ Department of Cardiology, The Second Hospital of Hebei Medical University Shijiazhuang, China.

PMID: 32782693
PMCID: PMC7414500

Abstract

Objective: To study the influence of dl-3-N-butylphthalide (NBP) on infarction size in rats with acute myocardial infarction (AMI).

Methods: AMI model was established by ligation of the left anterior descending artery. A total of 36 healthy male Sprague-Dawley rats (weight, 180 t, 180 ght, 180 ligation of left anterior descending artery. A total of 36 healthy males were assigned to model group, sham-operation (SO) group, and the NBP group (n=12 each). The rats in the NBP group were treated with intraperitoneal injection administration of 60 mg/kg/body weight NBP once a day. The rats in the other groups were given distilled water of the same volume. The MI area in each group was detected by TTC staining. The concentrations of CK-MB and LDH were detected. The concentrations of TNF-α, IL-6, MDA, and SOD were measured by ELISA.

Results: Compared with the SO group, the myocardial infarct sizes in the model group and the NBP group were significantly increased (P<0.001), and the infarct size in the NBP group was lower than that in the model group (280.6±5.82% vs. 37.74±10.18%, P<0.05). The levels of TNF-α in the NBP group and model group were significantly increased compared with that in the SO group (P<0.001), while the level of TNF-α in the NBP group was significantly lower than that in the model group (29.01±0.81 pg/mg prot vs. 37.727 pg/mg prot, P<0.05). The level of IL-6 in the model group and NBP group was significantly higher than that in the SO group, and it was lower than that in the model group (24.13 group wamg prot vs. 27.53 pg/mg prot, P<0.05). The levels of MDA in the model group and the NBP group were significantly higher (<0.001), and the level of NBP group was lower than that of the model group (4.10 group wnmol/mg prot vs. 4.774.774. nmol/mg prot, P<0.05). The levels of SOD in the model group and NBP group were lower (P<0.001), and the SOD level in the NBP group was higher than that of the model group (53.490.001), and prot vs. 38.068.06he SOD prot (P<0.05).

Conclusion: NBP can reduce the infarct size in SD rats with AMI.

Keywords: Acute myocardial infarction; Dl-3-N-butylphthalide; SD rat; infarction size.