Glucose photoelectrochemical enzyme sensor based on competitive reaction of ascorbic acid

Delun Chen; Xiaohong Wang; Kexi Zhang; Yang Cao; Jinchun Tu; Dan Xiao; Qiang Wu

doi:10.1016/j.bios.2020.112466

Glucose photoelectrochemical enzyme sensor based on competitive reaction of ascorbic acid

Biosens Bioelectron. 2020 Oct 15:166:112466. doi: 10.1016/j.bios.2020.112466. Epub 2020 Jul 27.

Authors

Delun Chen¹, Xiaohong Wang², Kexi Zhang², Yang Cao³, Jinchun Tu⁴, Dan Xiao⁵, Qiang Wu⁶

Affiliations

¹ School of Tropical Medicine and Laboratory Medicine, Key Laboratory of Emergency and Trauma of Ministry of Education, Research Unit of Island Emergency Medicine, Chinese Academy of Medical Sciences (No. 2019RU013), Hainan Medical University, Haikou, 571199, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.
² State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.
³ State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; Qiongtai Normal University, Haikou, 571127, China.
⁴ State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China. Electronic address: tujinchun@hainu.edu.cn.
⁵ Key Laboratory of Green and Technology, Ministry of Education, College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu, 610065, China. Electronic address: xiaodan@scu.edu.cn.
⁶ School of Tropical Medicine and Laboratory Medicine, Key Laboratory of Emergency and Trauma of Ministry of Education, Research Unit of Island Emergency Medicine, Chinese Academy of Medical Sciences (No. 2019RU013), Hainan Medical University, Haikou, 571199, China. Electronic address: wuqiang001001@aliyun.com.

PMID: 32777725
DOI: 10.1016/j.bios.2020.112466

Abstract

A new method based on the competitive reaction of ascorbic acid (AA) was used to improving the performance of photoelectrochemical glucose enzyme sensor. In this method, amplifying the photoelectric signal of H₂O₂ by the competitive reaction of AA is the key step. The detection can be well operated at 0 V under optimal AA concentration of 10 mM. In the method, AA was regarded as not only the electron donor to capture the hole in the conduction band of ZnO, but also the remover of H₂O₂ produced by the oxidation of glucose. Both these factors led to the formation of a pair of competitive reactions that enhanced the response towards glucose detection. Compared to the detection without AA, the stability of the response current, detection ranges of 1-19 mM, detection limit of 80 μM and sensitivity of 2.88 μA mM^-1·cm^-2 were optimized prominently.

Keywords: Competitive reaction; Photoelectrochemical enzyme sensor; ZnO.

MeSH terms

Ascorbic Acid*
Biosensing Techniques*
Glucose
Glucose Oxidase
Hydrogen Peroxide

Substances

Hydrogen Peroxide
Glucose Oxidase
Glucose
Ascorbic Acid