Cyclin F and KIF20A, FOXM1 target genes, increase proliferation and invasion of ovarian cancer cells

Exp Cell Res. 2020 Oct 15;395(2):112212. doi: 10.1016/j.yexcr.2020.112212. Epub 2020 Aug 7.

Abstract

Increased expression of FOXM1 is observed in a variety of human malignancies. The downstream target genes of FOXM1 involved in tumorigenesis and development are not fully elucidated in ovarian cancer. Here, we identified Cyclin F, a substrate recognition subunit of SCF (Skp1-Cul1-F-box protein) complex, and Kinesin Family Member 20A (KIF20A) were transcriptionally regulated by FOXM1 in ovarian cancer. Accordingly, Cyclin F and KIF20A were commonly overexpressed in ovarian cancer. Functionally, forced expression of Cyclin F or KIF20A significantly enhanced while knockdown of them decreased proliferation and invasion of ovarian cancer cells. Importantly, high levels of Cyclin F and KIF20A correlated with poor prognosis in patients with ovarian cancer. Our findings indicate that Cyclin F and KIF20A are functional targets of FOXM1 which might be potential drug targets in ovarian cancer.

Keywords: Cyclin F; FOXM1; KIF20A; Ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics
  • Carcinoma, Ovarian Epithelial / genetics*
  • Cell Movement / physiology
  • Cell Proliferation / genetics*
  • Cell Transformation, Neoplastic / genetics
  • Cyclins / metabolism*
  • Female
  • Forkhead Box Protein M1 / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kinesins / genetics*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology
  • Ovary / metabolism
  • Ovary / pathology

Substances

  • Biomarkers, Tumor
  • CCNF protein, human
  • Cyclins
  • FOXM1 protein, human
  • Forkhead Box Protein M1
  • KIF20A protein, human
  • Kinesins