An ATP binding cassette transporter HvABCB25 confers aluminum detoxification in wild barley

Wenxing Liu; Xue Feng; Fangbin Cao; Dezhi Wu; Guoping Zhang; Eva Vincze; Yizhou Wang; Zhong-Hua Chen; Feibo Wu

doi:10.1016/j.jhazmat.2020.123371

An ATP binding cassette transporter HvABCB25 confers aluminum detoxification in wild barley

J Hazard Mater. 2021 Jan 5:401:123371. doi: 10.1016/j.jhazmat.2020.123371. Epub 2020 Jul 3.

Authors

Wenxing Liu¹, Xue Feng¹, Fangbin Cao², Dezhi Wu¹, Guoping Zhang¹, Eva Vincze³, Yizhou Wang¹, Zhong-Hua Chen⁴, Feibo Wu⁵

Affiliations

¹ Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
² Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China.
³ Department of Molecular Biology and Genetics, University of Aarhus, Fosøgsvej 1, DK-4200 Slagelse, Denmark.
⁴ School of Science, Western Sydney University, Penrith, NSW 2751, Australia; Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW 2751, Australia. Electronic address: Z.Chen@westernsydney.edu.au.
⁵ Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China. Electronic address: wufeibo@zju.edu.cn.

PMID: 32763683
DOI: 10.1016/j.jhazmat.2020.123371

Abstract

Aluminum (Al) stress in acid soils is one of the major factors limiting crop productivity. ATP binding cassette (ABC) transporters have numerous roles in plants, but the link between ABCB protein subfamily and plant Al tolerance is still elusive. Here, we identiﬁed and characterized a novel tonoplast HvABCB25 in barley root cells. HvABCB25 was up-regulated in the transcriptome of Al-tolerant wild barley XZ16 under Al treatment and was highly Al-inducible in root tips. ABCB25 is originated from Streptophyte algae and evolutionarily conserved in land plants. Moreover, silencing HvABCB25 in Al-tolerant XZ16 led to significant suppression of Al tolerance as indicated by significantly reduced root growth and enhanced Al accumulation in root cells. Conversely, HvABCB25-overexpressed plants and Golden Promise showed similar Al content in whole roots and in cell sap, but the overexpression lines exhibited significantly higher Al-induced relative root growth and dry weight. Al florescence in cytosol of root cells were significantly less in overexpression lines than that in GP. These results indicated that overexpressing HvABCB25 may be responsible for Al detoxification via vacuolar Al sequestration in barley roots, providing useful insight into the genetic basis for a new Al detoxification mechanism towards plant Al tolerance in acid soils.

Keywords: ABC transporter; Al-tolerance; Hordeum spontaneum; Internal detoxification; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters
Aluminum / metabolism
Aluminum / toxicity
Gene Expression Regulation, Plant
Hordeum* / genetics
Hordeum* / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Roots / genetics
Plant Roots / metabolism
Vacuoles / metabolism

Substances

ATP-Binding Cassette Transporters
Plant Proteins
Aluminum