Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Binna Lv; Na Jiang; Rakibul Hasan; Yingying Chen; Manhong Sun; Shidong Li

doi:10.3389/fmicb.2020.01640

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Front Microbiol. 2020 Jul 15:11:1640. doi: 10.3389/fmicb.2020.01640. eCollection 2020.

Authors

Binna Lv¹, Na Jiang¹, Rakibul Hasan¹, Yingying Chen¹, Manhong Sun¹, Shidong Li¹

Affiliation

¹ Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Abstract

Cell wall biogenesis protein phosphatases play important roles in various cellular processes in fungi. However, their functions in the widely distributed mycoparasitic fungus Clonostachys rosea remain unclear, as do their potential for controlling plant fungal diseases. Herein, the function of cell wall biogenesis protein phosphatase CrSsd1 in C. rosea 67-1 was investigated using gene disruption and complementation approaches. The gene-deficient mutant ΔCrSsd1 exhibited much lower conidiation, hyphal growth, mycoparasitic ability, and biocontrol efficacy than the wild-type (WT) strain, and it was more sensitive to sorbitol and Congo red. The results indicate that CrSsd1 is involved in fungal conidiation, osmotic stress adaptation, cell wall integrity, and mycoparasitism in C. rosea.

Keywords: Clonostachys rosea; Ssd1; biocontrol efficacy; cell wall integrity; conidiation; mycoparasitism.