Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip

Xi Huang; Xinyu Huang; Jianhua Xie; Xiujiang Li; Zhibing Huang

doi:10.1016/j.ab.2020.113878

Rapid simultaneous detection of fumonisin B₁ and deoxynivalenol in grain by immunochromatographic test strip

Anal Biochem. 2020 Oct 1:606:113878. doi: 10.1016/j.ab.2020.113878. Epub 2020 Aug 2.

Authors

Xi Huang¹, Xinyu Huang¹, Jianhua Xie², Xiujiang Li³, Zhibing Huang⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China.
³ The First Affiliated Hospital of Nanchang University, Nanchang University, No.17 Yongwai Main Street, Nanjing West Road, Nanchang, 330006, China.
⁴ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China. Electronic address: hzbchem@ncu.edu.cn.

PMID: 32755601
DOI: 10.1016/j.ab.2020.113878

Abstract

Mycotoxins, such as fumonisin B₁ (FB₁) and deoxynivalenol (DON), are toxic secondary metabolites produced by fungi. Herein, the simultaneous detection of FB₁ and DON (both of which are water-soluble) in grain samples by immunochromatographic methods was used to probe whether the levels of these mycotoxins exceeded the limit of 1 mg/kg. Two types of immunochromatographic test strips (ICSs) were developed for this purpose, namely, those based on Au nanospheres (prepared via reduction with trisodium citrate) and Au nanoflowers (prepared by Au seed-mediated growth) as markers, with the respective sensitivities to both FB₁ and DON equalling 20 and 5.0 ng/mL. The former ICS was used to detect FB₁ and DON in grain (51 wheat samples and 18 maize samples), providing results consistent with those of high-performance liquid chromatography and enzyme-linked immunosorbent assays. The developed technique was suitable for the on-site screening of large-scale samples for FB₁ and DON.

Keywords: Deoxynivalenol; Fumonisin B(1); Grain; Immunochromatographic test strip.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry
Chromatography, Affinity / instrumentation
Chromatography, Affinity / methods*
Edible Grain / chemistry*
Edible Grain / microbiology
Food Contamination / analysis*
Fumonisins / analysis*
Gold Colloid / chemistry
Limit of Detection
Metal Nanoparticles / chemistry
Mycotoxins / analysis
Reagent Strips
Trichothecenes / analysis*
Zea mays / chemistry
Zea mays / microbiology

Substances

Antibodies, Monoclonal
Fumonisins
Gold Colloid
Mycotoxins
Reagent Strips
Trichothecenes
fumonisin B1
deoxynivalenol