Impact of centrifugation treatment on enzymatic hydrolysis of cellulose and xylan in poplar fibers with high lignin content

Abstract

Centrifugation is very common in the production and treatment of lignocellulose for applications like pretreatment for enzymatic hydrolysis, but it is not certain whether it affects applications of lignocellulose and almost no one realizes this problem. This study investigated the effects of centrifugation on the characteristics and enzymatic hydrolysis of poplar fibers with high lignin content. The results showed that centrifugation inhibited the enzymatic hydrolysis of fiber, but fiber characteristics and enzymatic digestibility fluctuated with increasing centrifugation time. Centrifugation for about 15 min had the least effect on fiber properties while centrifugation for 30 min had the least effect on enzymatic hydrolysis. The water retention value was closely related to the enzymatic digestibility, but the pore characteristics and crystallinity index could not reflect the enzymatic accessibility of the fiber. This article will provide useful references for the enzymatic hydrolysis of lignocellulose and other high-value applications to improve production efficiency furtherly.

Keywords: Centrifugation treatment; Characteristics; Enzymatic hydrolysis; Inhibition; Lignocellulose.