Facile Synthesis of Enzyme-Embedded Metal-Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Yangxin Wang; Ningning Zhang; Deming Tan; Zhenhui Qi; Changzhu Wu

doi:10.3389/fbioe.2020.00714

Facile Synthesis of Enzyme-Embedded Metal-Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Front Bioeng Biotechnol. 2020 Jul 7:8:714. doi: 10.3389/fbioe.2020.00714. eCollection 2020.

Authors

Yangxin Wang^{1

2

3}, Ningning Zhang³, Deming Tan⁴, Zhenhui Qi², Changzhu Wu^{5

6}

Affiliations

¹ College of Materials Science and Engineering, Nanjing Tech University, Nanjing, China.
² Sino-German Joint Research Lab for Space Biomaterials and Translational Technology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.
³ Institute of Microbiology, Technische Universität Dresden, Dresden, Germany.
⁴ Department of Chemistry, Technische Universität Dresden, Dresden, Germany.
⁵ Department of Physics, Chemistry and Pharmacy University of Southern Denmark, Odense, Denmark.
⁶ Danish Institute for Advanced Study, University of Southern Denmark, Odense, Denmark.

Abstract

In situ immobilization of enzyme into metal-organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO₃)₂⋅6H₂O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

Keywords: biocatalysis; enzymatic reactions; heterogeneous catalysis; metal–organic frameworks; size-selective catalysis.