A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

Zemiao Xia; Haopeng Geng; Yuan Cai; Yaonan Wang; Daquan Sun; Jian Zhang; Zhiming Pan; Xin'an Jiao; Shizhong Geng

doi:10.3389/fvets.2020.00324

A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

Front Vet Sci. 2020 Jul 3:7:324. doi: 10.3389/fvets.2020.00324. eCollection 2020.

Authors

Zemiao Xia^{1

2

3

4}, Haopeng Geng^{1

2

3

4}, Yuan Cai^{1

2

3

4}, Yaonan Wang^{1

2

3

4}, Daquan Sun^{1

2

3

4}, Jian Zhang^{1

2

3

4}, Zhiming Pan^{1

2

3

4}, Xin'an Jiao^{1

2

3

4}, Shizhong Geng^{1

2

3

4}

Affiliations

¹ Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
² Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China.
³ Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, China.
⁴ Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, China.

Abstract

Salmonella enteritidis and Salmonella pullorum belonging to Group O₉ Salmonella are major causative agents of infectious diseases in chicken. O₉ antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for Salmonella infection. To identify the infection, an anti-O₉ monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O₉ Dc-ELISA) was developed after constraints were optimized; the establishment and application of O₉ Dc-ELISA, compared to two commercial kits and plate agglutination test (PAT), showed that O₉ Dc-ELISA could screen out more positive samples than the PAT method could and produce the same agreement rates with commercial kits in terms of sensitivity in addition to strong specificity to clinical serum samples.

Keywords: McAb; O9 Dc-ELISA; O:9 Salmonella; PAT; specificity.