Proteomic analysis in different development stages on SP0 generation of rice seeds after space flight

Deyong Zeng; Jie Cui; Yishu Yin; Meng Zhang; Shan Shan; Meng Yao Liu; Dayou Cheng; Weihong Lu; Yeqing Sun

doi:10.1016/j.lssr.2020.02.001

Proteomic analysis in different development stages on SP0 generation of rice seeds after space flight

Life Sci Space Res (Amst). 2020 Aug:26:34-45. doi: 10.1016/j.lssr.2020.02.001. Epub 2020 Mar 5.

Authors

Deyong Zeng¹, Jie Cui², Yishu Yin³, Meng Zhang⁴, Shan Shan⁵, Meng Yao Liu⁶, Dayou Cheng⁷, Weihong Lu⁸, Yeqing Sun⁹

Affiliations

¹ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: 18B925086@stu.hit.edu.cn.
² School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: cuijie2006@163.com.
³ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: 18S025092@stu.hit.edu.cn.
⁴ Environment System Biological Institute, Dalian Maritime University, Dalian 116026, China. Electronic address: zmdlmu@dlmu.edu.cn.
⁵ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: 925035@hit.edu.cn.
⁶ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: 009720661@qq.com.
⁷ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: hgdcdy@126.com.
⁸ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China. Electronic address: lwh@hit.edu.cn.
⁹ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China; Environment System Biological Institute, Dalian Maritime University, Dalian 116026, China. Electronic address: yqsun@dlmu.edu.cn.

PMID: 32718685
DOI: 10.1016/j.lssr.2020.02.001

Abstract

The space biological effects of plants will drive the development of aerospace science and breeding science. The aim of this study is to reveal changes in the proteome of contemporary plants at different growth and development stages after space flight of rice seeds. We carried the rice seeds (DN416) through the SJ-10 returning satellite and returned to the ground for planting to the three-leaf stage (TLP) and tillering stage (TS) after a 12.5-day orbital flight. We found that the space flight caused the rice germination rate, the TLP plant height, and the number of tillers in the TS decreased by 11.64%, 9.75%, and 9.80%, respectively. In addition, the treatment group ROS and MDA level increased in the TLP and TS. The abundance patterns of proteins in these leaves identified 214 proteins in the TLP and 286 in the TS leaves that were markedly changed. Moreover, our study identified D14 proteins that control plant height and tiller. Our results show that the space environment may affect the downstream signaling mechanism by regulating the level of ROS in the body to achieve a response to the space environment. Meanwhile, the space environment may affect the plant height and tiller of rice by altering the expression of D14 protein and hormone-regulated proteins. Our results reveal changes in the proteome of different growth stages of rice plants, and also reveal the molecular mechanism of space environment regulation of rice plant height and tiller, which provides a new direction for further understanding of space biological effects and space mutation breeding.

Keywords: Proteomic; Rice; SJ-10 returning satellite; space flight.

MeSH terms

Germination*
Oryza / growth & development*
Plant Proteins / metabolism*
Proteome / metabolism*
Proteomics
Seeds / growth & development*
Space Flight*

Substances

Plant Proteins
Proteome