Inhibiting Cell Viability and Motility by Layer-by-Layer Assembly and Biomineralization

Yan Wei; Hao Xu; Shuangmeng Xu; Hui Su; Lichuang Zhang; Ruize Sun; Di Huang; Liqin Zhao; Kaiqun Wang; Yinchun Hu; Xiaojie Lian

doi:10.1021/acsomega.0c00846

Inhibiting Cell Viability and Motility by Layer-by-Layer Assembly and Biomineralization

ACS Omega. 2020 Jul 10;5(28):17118-17128. doi: 10.1021/acsomega.0c00846. eCollection 2020 Jul 21.

Authors

Yan Wei¹, Hao Xu¹, Shuangmeng Xu¹, Hui Su¹, Lichuang Zhang¹, Ruize Sun¹, Di Huang¹, Liqin Zhao¹, Kaiqun Wang¹, Yinchun Hu¹, Xiaojie Lian¹

Affiliation

¹ Department of Biomedical Engineering, Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.

Abstract

Herein, we proposed a drug-free strategy named cell surface shellization to inhibit the motility of SKOV-3 and HeLa cells. We alternately deposited two- or three-layer cationic polyelectrolyte (PE) and anionic PE films on the surface of SKOV-3 and HeLa cells. Then, a mineral shell (calcium carbonate, CaCO₃) was formed on the surface of polymer shells via electrostatic force and biomineralization. The CCK-8 assay results and live/dead staining showed that the surface shells strongly aggravated the cytotoxicity. The monolayer scratch wound migration assay results and immunofluorescence staining results showed that the shells, especially the mineral shells, could efficiently inhibit the migration of SKOV-3 and HeLa cells without any anticancer drugs. The immunofluorescence results of the three small G proteins of the cells showed that the immunofluorescence intensity in SKOV-3 did not change. Preliminary results from our laboratory showed an increase in MMP-9 secreted by cancer cells after coating with films or mineral shells. It suggests that mechanisms that inhibit cell migration are related to the MMP signaling pathway. All the results indicated that shellization (films or nanomineral shells) but not limited to calcification can be used as one of the tools to change the function of cells.