Synthesis of silver nanoparticles prepared with a dextran-type exopolysaccharide from Weissella cibaria MED17 with antimicrobial functions

Prep Biochem Biotechnol. 2021;51(2):112-119. doi: 10.1080/10826068.2020.1795673. Epub 2020 Jul 25.

Abstract

Microbial polysaccharides can find distinct applications as stabilizer agents including synthesis of nanoparticles. In this study, a dextran-type exopolysaccharide (EPS) was used as the stabilizer agent for the green synthesis of silver nanoparticles (AgNPs-Dex) with antimicrobial characteristics. UV-vis spectroscopy analysis was used to test the formation of AgNPs-Dex. The uniform morphology at around 10 nm size was observed for AgNPs-Dex by TEM analysis and importantly EDX analysis demonstrated the embedment of Ag+ ions within dextran as the stabilizer agent. XRD analysis confirmed the crystalline nature of AgNPs-Dex and FTIR analysis demonstrated the interactions of dextran functional groups with silver. DSC and TGA analysis showed the alteration in the thermal stability of AgNPs-Dex compared to the stabilizer dextran. The antibacterial and antifungal activities of AgNPs-Dex were determined against food originated pathogenic bacteria and fungi and important inhibition levels were observed at 1 mg ml-1 concentration of AgNPs-Dex and this activity was observed to be concentration dependent.

Keywords: Antibacterial; antifungal; dextran; silver nanoparticles.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Infective Agents / pharmacology
  • Antifungal Agents / chemistry
  • Bacteria / drug effects
  • Dextrans / chemistry*
  • Ions
  • Metal Nanoparticles / chemistry*
  • Microbial Sensitivity Tests
  • Nanoparticles
  • Plant Extracts / chemistry
  • Polysaccharides / chemistry*
  • Silver / chemistry*
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Stress, Mechanical
  • Temperature
  • Thermogravimetry
  • Weissella / metabolism*
  • X-Ray Diffraction

Substances

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Antifungal Agents
  • Dextrans
  • Ions
  • Plant Extracts
  • Polysaccharides
  • Silver

Supplementary concepts

  • Weissella cibaria