Target-site directed rational high-throughput screening system for high sophorolipids production by Candida bombicola

Yang Chen; Xiwei Tian; Qianhui Li; Ya Li; Ju Chu; Haifeng Hang; Yingping Zhuang

doi:10.1016/j.biortech.2020.123856

Target-site directed rational high-throughput screening system for high sophorolipids production by Candida bombicola

Bioresour Technol. 2020 Nov:315:123856. doi: 10.1016/j.biortech.2020.123856. Epub 2020 Jul 17.

Authors

Yang Chen¹, Xiwei Tian¹, Qianhui Li¹, Ya Li¹, Ju Chu¹, Haifeng Hang², Yingping Zhuang¹

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
² State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: hanghaifeng@ecust.edu.cn.

PMID: 32707507
DOI: 10.1016/j.biortech.2020.123856

Abstract

In this study we have established a rational and high performance high-throughput screening system to select for a sophorolipids (SLs) high-producing strain of Candida bombicola. Introduction of mutagen combination, relaxation culture and multi-stress significantly improved both mutation and positive mutation rates. A high-performing strain, Ncbio 5, was selected out of 6212 mutants. Final SLs titer, productivity and yield of this strain in 5 L bioreactors were 26.9%, 27.0% and 35.0% higher than in the original strain, respectively. The improved fermentation performance in Ncbio 5 is contributed by the longer production period, higher SLs productivity and more efficient oil utilization. The strategy adopted herein to optimize the high-throughput screening system should be readily extendable to other similar systems.

Keywords: Candida bombicola; Combined mutagenesis; Combined pressure; Relaxation culture; Sophorolipids.

MeSH terms

Candida*
Fermentation
Glycolipids*
Oleic Acids

Substances

Glycolipids
Oleic Acids
sophorolipid