Development of a rapid and accurate UHPLC-PDA-FL method for the quantification of phenolic compounds in grapes

Ana V González de Peredo; Mercedes Vázquez-Espinosa; Zulema Piñeiro; Estrella Espada-Bellido; Marta Ferreiro-González; Gerardo F Barbero; Miguel Palma

doi:10.1016/j.foodchem.2020.127569

Development of a rapid and accurate UHPLC-PDA-FL method for the quantification of phenolic compounds in grapes

Food Chem. 2021 Jan 1:334:127569. doi: 10.1016/j.foodchem.2020.127569. Epub 2020 Jul 18.

Authors

Ana V González de Peredo¹, Mercedes Vázquez-Espinosa¹, Zulema Piñeiro², Estrella Espada-Bellido¹, Marta Ferreiro-González¹, Gerardo F Barbero³, Miguel Palma¹

Affiliations

¹ Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, P.O. Box 40, 11510 Puerto Real, Cadiz, Spain.
² Centro IFAPA Rancho de la Merced, Carretera de Trebujena, km 3.2, Apdo. 589, 11471 Jerez de la Frontera, Cadiz, Spain.
³ Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, P.O. Box 40, 11510 Puerto Real, Cadiz, Spain. Electronic address: gerardo.fernandez@uca.es.

PMID: 32707360
DOI: 10.1016/j.foodchem.2020.127569

Abstract

Grapes are a great source of phenolic compounds, which have excellent antioxidant properties. Efficient analytical methods are necessary to selectively and precisely determine these compounds content in grapes. In this study, a reverse-phase ultra-high performance liquid chromatography (UHPLC) method with fluorescence and photodiode array detection has been developed to determine and quantify 27 of the main phenolic compounds present in grapes. An ACQUITY UPLC® BEH C18 (50 mm × 2.1 mm i.d., 1.7 mm particle size) column was employed. A gradient method was developed and column temperature (25-55 °C), as well as flow rate (0.6-0.75 mL min^-1), were optimized. The optimum conditions allowed the separation of all the compounds in less than 9 min. The method was validated and demonstrated excellent detection and quantification limits, precision, and selectivity. Finally, several grape varieties were studied in order to demonstrate the applicability of the method to the analysis of real matrix samples.

Keywords: Flavonoids; Fluorescence; Grapes; PDA; Phenolic compounds; UHPLC.

MeSH terms

Chromatography, High Pressure Liquid / methods*
Flavonoids / analysis*
Flavonoids / isolation & purification
Fruit / chemistry*
Limit of Detection
Phenols / analysis
Phenols / isolation & purification
Sensitivity and Specificity
Vitis / chemistry*

Substances

Flavonoids
Phenols