Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran

Taher Azimi; Mohammad Reza Pourmand; Fatemeh Fallah; Abdollah Karimi; Roxana Mansour-Ghanaie; Seyedeh Mahsan Hoseini-Alfatemi; Mehdi Shirdoust; Leila Azimi

doi:10.1186/s41182-020-00246-3

Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran

Trop Med Health. 2020 Jul 22:48:60. doi: 10.1186/s41182-020-00246-3. eCollection 2020.

Authors

Taher Azimi^{1

2}, Mohammad Reza Pourmand¹, Fatemeh Fallah³, Abdollah Karimi³, Roxana Mansour-Ghanaie³, Seyedeh Mahsan Hoseini-Alfatemi³, Mehdi Shirdoust³, Leila Azimi³

Affiliations

¹ Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
² Students Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
³ Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, P.O. Box 15468-15514, Tehran, Iran.

Abstract

Background: Rattus norvegicus are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of R. norvegicus throughout the city of Tehran, this study aims to assess the frequency of zoonotic parasites carried by commensal rodents wandering in Tehran, Iran. The study considered the north, south, west, east, and center regions of Tehran for the purposes of this study. The serological tests were applied in order to detect effective antibodies against Trichomonas vaginalis (T. vaginalis), Babesia spp., and Cryptosporidium spp. using a commercial qualitative rat ELISA kit. The frequency of Toxoplasma gondii (T. gondii) was surveyed by using the conventional PCR method. Furthermore, nested PCR was employed to detect the presence of Giardia spp. and Leishmania spp. in commensal R. norvegicus dispersed in Tehran.

Results: Approximately, 76% of the 100 R. norvegicus tested were infected with at least one zoonotic parasite, indicating the significant frequency of parasites within the study areas. Seroreactivity against T. vaginalis, Babesia spp., and Cryptosporidium spp. was detected in 5%, 0%, and 1% of the R. norvegicus tested, respectively. T. gondii DNA was detected in 32 out of 100 (32%) R. norvegicus. In addition, Leishmania spp. and Giardia spp. DNA were found in 18 out of 100 (18%) and 76 out of 100 (76%) R. norvegicus investigated, respectively. T. vaginalis with 15% and T. gondii with 70% had the highest frequency of parasites among the R. norvegicus collected from the western and northeastern regions of Tehran, respectively. Moreover, Giardia spp. with 95% and Leishmania spp. with 30% had the highest frequency in the east and center districts, respectively.

Conclusion: The findings showed a wide geographical dissemination of Giardia spp., Toxoplasma gondii, and Leishmania spp. in R. norvegicus within five districts of Tehran. In contrast, other parasites such as Cryptosporidium spp. infection were rarely detected in Rattus populations. No evidence for the circulation of Babesia spp. was found in this study.

Keywords: Giardia spp.; Leishmania spp.; Rattus norvegicus; Tehran; Toxoplasma gondii; Zoonotic parasites.