Abstract
A probe that allows specific 'painting' of human tumours is described. Probe activation was mediated by specific matrix metalloproteinases, resulting not only in disruption of a FRET pair, but in the generation of a fragment that "fluorescently paints" human tumours. This probe demonstrated rapid and effective human tumour labelling with the potential to allow margin detection during surgical resection.
MeSH terms
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Carbocyanines / chemistry
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Fluoresceins / chemistry
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Fluorescent Dyes / chemistry*
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Humans
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Matrix Metalloproteinases / chemistry
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Matrix Metalloproteinases / metabolism*
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Microscopy, Fluorescence
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Neoplasms / metabolism
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Neoplasms / pathology*
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Peptides / chemical synthesis
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Peptides / metabolism
Substances
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CY5.5 cyanine dye
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Carbocyanines
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Fluoresceins
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Fluorescent Dyes
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Peptides
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4-carboxyfluorescein
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Matrix Metalloproteinases