Huoxin Pill Attenuates Cardiac Inflammation by Suppression of TLR4/NF- κ B in Acute Myocardial Ischemia Injury Rats

Jianfeng Chu; Xueling Zhou; Meizhong Peng; Yan Lu; Ali Farman; Lianli Peng; Huajian Gao; Qi Li; Xintong Chen; Lingling Xie; Youqin Chen; Aling Shen; Jun Peng

doi:10.1155/2020/7905902

Huoxin Pill Attenuates Cardiac Inflammation by Suppression of TLR4/NF- κ B in Acute Myocardial Ischemia Injury Rats

Evid Based Complement Alternat Med. 2020 Jul 9:2020:7905902. doi: 10.1155/2020/7905902. eCollection 2020.

Authors

Jianfeng Chu^{1

2

3}, Xueling Zhou^{1

2

3}, Meizhong Peng^{1

2

3}, Yan Lu^{1

2

3}, Ali Farman^{1

2

3}, Lianli Peng⁴, Huajian Gao^{1

2

3}, Qi Li⁵, Xintong Chen^{1

2

3}, Lingling Xie^{1

2

3}, Youqin Chen^{1

2

3

6}, Aling Shen^{1

2

3}, Jun Peng^{1

2

3}

Affiliations

¹ Academy of Integrative Medicine, Fuzhou, Fujian 350122, China.
² Chen Keji Academic Thought Inheritance Studio, Fuzhou, Fujian 350122, China.
³ Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
⁴ College of Engineering and Computer Science, The Australian National University, Canberra, ACT 2600, Australia.
⁵ Department of Research and Development, Youcare Pharmaceutical Group Co., Ltd., Beijing 100176, China.
⁶ Department of Pediatric Gastroenterology, Rainbow Babies & Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Abstract

Huoxin Pill (HXP), a traditional Chinese medicine, has been prescribed widely in the treatment of coronary heart disease, angina pectoris, and other diseases. However, the possible protective mechanisms of HXP on myocardial ischemia remain unclear. In the current study, we investigated the effects and potential mechanism of HXP on myocardial ischemia and cardiac inflammation and the activation of TLR4/NF-κB pathway. Determination of electrocardiogram, echocardiography, and heart weight index (HWI) indicated that HXP treatment obviously attenuated the elevation of ST-segment, end-diastolic volume, and HWI in the AMI rat model. Enzyme-linked immunosorbent assay (ELISA) demonstrated that Huoxin Pill treatment significantly decreased the levels of CTnT, CK-MB, MDA, IL-6, and TNF-α, while it increased SOD content in serum of the AMI rat model. Moreover, hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining revealed that HXP treatment alleviated pathological change, infiltration of inflammatory cells, levels of IL-6 and TNF-α, and expression of TLR4 and p-NF-κB in cardiac tissues of the AMI rat model. In conclusion, HXP treatment significantly improves cardiac function and attenuates cardiac inflammation by suppressing the activation of TLR4/NF-κB pathway in the ISO-induced AMI rat model. This study provides insights into the potential of HXP on prevention and treatment of AMI.