Electrochemiluminescence immunosensor for tropomyosin using carbon nanohorns/Nafion/Fe3O4@Pd screen-printed electrodes

Mikrochim Acta. 2020 Jul 18;187(8):456. doi: 10.1007/s00604-020-04440-2.

Abstract

This study focuses on developing a highly sensitive immunosensor by immobilizing oxidized carbon nanohorns/Nafion/Fe3O4@Pd nanocomposite on carbon screen-printed electrodes (SPEs) for the detection of tropomyosin (Tro-Ag). The performance of the fabricated immunosensor was investigated via electrochemiluminescence (ECL) method, resulting from the chemical reaction between tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)3]Cl2) and tripropylamine (TPrA), and the peak intensity is recorded at 1.0 V. The nanocomposite is able to enhance the ECL intensity of [Ru(bpy)3]2 +/TPrA system and achieves high sensitivity of 28.16 fg/mL with a dynamic working range of 28.16 fg/mL to 100 ng/ml.. Furthermore, the immunosensor demonstrated a decent stability and good repeatability for Tro-Ag detection in food products. Graphical abstract The schematic representation of the modified carbon SPE with CNHs-OH/Nafion/Fe3O4@Pd and the signal produced in the (a) absence and (b) presence of Tro-Ag.

Keywords: Carbon nanohorns; Electrochemiluminescence; Food allergen; Screen-printed electrode; Tropomyosin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Immobilized / immunology
  • Carbon / chemistry
  • Coordination Complexes / chemistry
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Electrodes
  • Ferrosoferric Oxide / chemistry
  • Fluorocarbon Polymers / chemistry
  • Immunoassay / methods*
  • Luminescent Measurements / methods*
  • Nanocomposites / chemistry*
  • Organometallic Compounds / chemistry
  • Palladium / chemistry
  • Penaeidae / chemistry
  • Propylamines / chemistry
  • Rabbits
  • Tropomyosin / analysis*
  • Tropomyosin / immunology

Substances

  • Antibodies, Immobilized
  • Coordination Complexes
  • Fluorocarbon Polymers
  • Organometallic Compounds
  • Propylamines
  • Tropomyosin
  • tris(2,2'-bipyridyl)ruthenium(II)
  • tris(2,2-bipyridine)-ruthenium(II)
  • tripropylamine
  • perfluorosulfonic acid
  • Palladium
  • Carbon
  • Ferrosoferric Oxide