Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction

Mônica Ghislaine Oliveira Alves; Natália da Silva Miguel; Camila Cristina Panisello Ferreira; Elis Ribeiro Alvarenga; Bruna Manzanares Tonon; Patrícia Pimentel de Barros; Celina Faig Lima Carta; Maria Beatriz Nogueira Paschoal; José Francisco de Sales Chagas; Celso Muller Bandeira; Fábio Daumas Nunes; Janete Dias Almeida

doi:10.1016/j.oooo.2020.06.003

Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction

Oral Surg Oral Med Oral Pathol Oral Radiol. 2020 Sep;130(3):298-305. doi: 10.1016/j.oooo.2020.06.003. Epub 2020 Jun 10.

Affiliations

¹ School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil; School of Dentistry, Brazcubas Educação, Mogi das Cruzes, Brazil; School of Medicine, Anhembi Morumbi University, São José dos Campos, Brazil. Electronic address: mgoliveiraalves@gmail.com.
² School of Dentistry, Brazcubas Educação, Mogi das Cruzes, Brazil.
³ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.
⁴ School of Dentistry, Brazcubas Educação, Mogi das Cruzes, Brazil; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.
⁵ Department of Head and Neck Surgery, São Leopoldo Mandic Medical School, Campinas, São Paulo, Brazil; Department of Head and Neck Surgery, Hospital Municipal Dr. Mário Gatti, Campinas, São Paulo, Brazil.
⁶ Department of Head and Neck Surgery, Hospital Municipal Dr. Mário Gatti, Campinas, São Paulo, Brazil.
⁷ Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil.

PMID: 32682592
DOI: 10.1016/j.oooo.2020.06.003

Abstract

Objective: The aim of this study was to evaluate the expression of DNA repair genes in cases of oral squamous cell carcinoma (OSCC).

Study design: Expression of the MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was evaluated by reverse transcription-quantitative polymerase chain reaction in the OSCC group (32 patients) and the control group (15 patients). The groups were compared by using the Mann-Whitney test, with Bonferroni correction. Associations between gene expression levels and clinical data were explored by using Pearson's and Spearman's correlation coefficients, with P value less than .05 indicating a significant difference.

Results: The MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes were downregulated in the OSCC group compared with the control group, with significant values for MLH1 (P < .0001); MSH2 (P = .038); MLH3 (P < .0001); ATM (P < .0001); MRE11A (P < .0001); XRCC1 (P = .0004); and PMS2 (P = .008). Analysis of the correlation between gene expression and clinical data only revealed a significant negative correlation between age and expression of the PMS2 gene.

Conclusions: Expression of the DNA repair genes MLH1, MSH2, MLH3, ATM, MRE11 AMRE11A, XRCC1, and PMS2 was reduced in OSCC.

MeSH terms

Carcinoma, Squamous Cell* / genetics
DNA Repair / genetics
DNA Repair Enzymes / metabolism
Humans
Mismatch Repair Endonuclease PMS2
Mouth Neoplasms* / genetics
MutL Protein Homolog 1 / genetics
MutL Protein Homolog 1 / metabolism
Polymerase Chain Reaction
Reverse Transcription
X-ray Repair Cross Complementing Protein 1

Substances

X-ray Repair Cross Complementing Protein 1
XRCC1 protein, human
Mismatch Repair Endonuclease PMS2
MutL Protein Homolog 1
DNA Repair Enzymes