Proteomics-Based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA

Kyoko Hiragami-Hamada; Naoki Tani; Jun-Ichi Nakayama

doi:10.1007/978-1-0716-0680-3_8

Proteomics-Based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA

Methods Mol Biol. 2020:2161:89-99. doi: 10.1007/978-1-0716-0680-3_8.

Authors

Kyoko Hiragami-Hamada^{1

2}, Naoki Tani³, Jun-Ichi Nakayama^{4

5}

Affiliations

¹ Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Aichi, Japan. khamada@nibb.ac.jp.
² Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan. khamada@nibb.ac.jp.
³ Liaison Laboratory Research Promotion Center, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
⁴ Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Aichi, Japan.
⁵ Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan.

PMID: 32681508
DOI: 10.1007/978-1-0716-0680-3_8

Abstract

Chromatin serves as a platform for a multitude of biological processes, including transcription and co-transcriptional RNA processing. Consequently, chromatin is likely to be covered with many RNA molecules.Here we describe a simple, reliable, and cross-link-free method for the systematic identification of chromatin-associated RBPs that exhibit RNA-dependent chromatin association.

Keywords: Chromatin; Protein; RNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatin / chemistry
Chromatin / metabolism*
HeLa Cells
Humans
K562 Cells
Nuclear Proteins / chemistry
Nuclear Proteins / metabolism*
Protein Interaction Mapping / methods*
Proteomics / methods*
RNA / chemistry
RNA / metabolism*
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / metabolism*

Substances

Chromatin
Nuclear Proteins
RNA-Binding Proteins
RNA