Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

Beob Soo Kim; Mitsuru Naito; Rimpei Kamegawa; Hyun Jin Kim; Ryo Iizuka; Takashi Funatsu; Shingo Ueno; Takanori Ichiki; Akihiro Kishimura; Kanjiro Miyata

doi:10.1039/d0cc01750g

Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

Chem Commun (Camb). 2020 Aug 19;56(66):9477-9480. doi: 10.1039/d0cc01750g.

Authors

Affiliations

¹ Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. miyata@bmw.t.u-tokyo.ac.jp.
² Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
³ Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
⁴ Innovation Center of NanoMedicine, Kawasaki Institute of Inductrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kawasaki 210-0821, Japan.
⁵ Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. miyata@bmw.t.u-tokyo.ac.jp and Innovation Center of NanoMedicine, Kawasaki Institute of Inductrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kawasaki 210-0821, Japan.
⁶ Center for Molecular Systems, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.

PMID: 32677638
DOI: 10.1039/d0cc01750g

Abstract

A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells.

MeSH terms

A549 Cells
DNA Damage / drug effects
DNA Damage / radiation effects
Fluorescent Dyes / chemistry
Gene Knockdown Techniques / methods*
Humans
Microscopy, Confocal
Nanostructures / chemistry*
Nanostructures / toxicity
Oligodeoxyribonucleotides / chemistry*
Peptides / chemistry
Polyethylene Glycols / chemistry
Ultraviolet Rays*

Substances

Fluorescent Dyes
Oligodeoxyribonucleotides
Peptides
Polyethylene Glycols