The purpose of the present work is to fabricate emulsions with excellent stability to deliver β-carotene using a novel biomacromolecule. β-Lactoglobulin-ferulic acid-chitosan ternary conjugate (BFCC), which was synthesized based on the carbodiimide-mediated coupling reaction and laccase induction, was confirmed by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) spectra. Also, BFCC was used to stabilize β-carotene emulsions. The results indicated that the concentration of BFCC affected the physical stability of β-carotene emulsions. Compared with the emulsions stabilized by β-lactoglobulin (β-LG), chitosan-ferulic acid conjugate (CFC), and β-LG-CFC mixture (BFCM), the emulsion stabilized by BFCC exhibited better stability under various environmental stresses. Moreover, the emulsion stabilized by BFCC had higher β-carotene retention during storage at 25 and 55 °C or under ultraviolet (UV) light exposure. The knowledge acquired in the current research offered an effective way to develop novel biomacromolecular emulsifiers and could find potential in fabricating delivery systems for bioactive compounds with markedly enhanced physiochemical properties.
Keywords: chitosan−ferulic acid conjugate; laccase induction; physicochemical stability; β-carotene emulsion; β-lactoglobulin.