Taurine-Conjugated Mussel-Inspired Iron Oxide Nanoparticles with an Elongated Shape for Effective Delivery of Doxorubicin into the Tumor Cells

Nimisha Singh; Nadine Millot; Lionel Maurizi; Gérard Lizard; Rajender Kumar

doi:10.1021/acsomega.0c01747

Taurine-Conjugated Mussel-Inspired Iron Oxide Nanoparticles with an Elongated Shape for Effective Delivery of Doxorubicin into the Tumor Cells

ACS Omega. 2020 Jun 23;5(26):16165-16175. doi: 10.1021/acsomega.0c01747. eCollection 2020 Jul 7.

Authors

Nimisha Singh^{1

2}, Nadine Millot², Lionel Maurizi², Gérard Lizard³, Rajender Kumar^{1

4}

Affiliations

¹ Department of Applied Chemistry, S. V. National Institute of Technology, Surat 395007, Gujarat, India.
² Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS/Université Bourgogne Franche-Comté, 9 Av. A. Savary, BP 47 870, Dijon 21078, France.
³ Laboratory Bio-PeroxIL, EA7270, Université Bourgogne Franche-Comté/Inserm, 6 Bd Gabriel, Dijon 21000, France.
⁴ Department of Chemistry and Chemical Science, School of Physical and Material Sciences, Central University of Himachal Pradesh, Kangra, Himachal Pradesh 176215, India.

Abstract

Multifunctional iron oxide magnetic nanoparticles, among them nanorods, were prepared with a mussel-inspired polydopamine (pDA) surface coating agent for cancer therapeutics. Taurine, a free sulfur-containing ß amino acid, was grafted on the pDA at the iron oxide nanoparticle surface to enhance its biocompatibility and targeted delivery action. Doxorubicin (DOX), an anticancer drug, was loaded on the prepared nanovehicles with an entrapment efficiency of 70.1%. Drug release kinetics were then analyzed using UV-vis and fluorescence spectroscopies, suggesting the pH-responsive behavior of the developed nanovehicle. The developed system was then tested on PC-3 cell lines to check its cellular response. Confocal microscopy observations and (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) and Annexin V-FITC assays used to evaluate cell toxicity and apoptosis reveal a dose-dependent nature of nanorods and can overcome the side effects of using free DOX with a targeted action.