Gene-wide identification and expression analysis of the PMEI family genes in soybean (Glycine max)

Jingjing Wang; Lei Ling; He Cai; Changhong Guo

doi:10.1007/s13205-020-02328-9

Gene-wide identification and expression analysis of the PMEI family genes in soybean (Glycine max)

3 Biotech. 2020 Aug;10(8):335. doi: 10.1007/s13205-020-02328-9. Epub 2020 Jul 6.

Authors

Jingjing Wang^{1

2}, Lei Ling¹, He Cai³, Changhong Guo¹

Affiliations

¹ Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, No. 1 of Shida Road, Limin Development Zone, Harbin, 150025 China.
² Heilongjiang Vocational College of Biology Science and Technology, Harbin, China.
³ Mudanjiang Normal University, Mudanjiang, China.

Abstract

Pectin Methylesterase Inhibitors (PMEI) gene family is widely spread in plants and plays crucial roles in plant development as well as biotic and abiotic stress response. However, little information was known about the function of PMEI genes in soybean. Herein, we identified 170 PMEI genes in soybean. These PMEI genes were divided into four groups (I-IV) based on phylogenetic analysis, and they were unevenly distributed in 18 soybean chromosomes. Gene structures and motif pattern analyses revealed that the PMEI genes in the same group showed the same characteristics. For the GmPMEI genes, gene duplication events occurred broadly, 52 pairs tandem duplication events and 55 pairs segmental duplication events suggested that the GmPMEI genes had high homology. Besides, the PMEI genes presented different expression patterns in different tissues, while several of these genes were expressed only in flowers. Under the biotic and abiotic stresses, PMEI genes had significant positive impact on the tolerance ability of soybean, and the ABA-responsive elements and SA-responsive elements played vital roles in responding to a variety of stresses. This study provides insights into the evolution and potential functions of GmPMEIs.

Keywords: F. oxysporum; P. sojae; PMEI protein; Soybean.