Abstract
High-speed AFM revealed the conformational change of fused in sarcoma (FUS) from a compact to an extended structure upon binding of non-coding RNA, which is supposed to allow FUS to bind to CBP/p300 for transcriptional interference. Thus, a mechanistic insight into transcription regulation by FUS and non-coding RNA is provided.
MeSH terms
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Green Fluorescent Proteins / chemistry
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Intrinsically Disordered Proteins / chemistry
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Intrinsically Disordered Proteins / metabolism
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Maltose-Binding Proteins / chemistry
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Microscopy, Atomic Force
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Promoter Regions, Genetic
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Protein Binding
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Protein Conformation
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RNA, Untranslated / metabolism*
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RNA-Binding Protein FUS / chemistry
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RNA-Binding Protein FUS / metabolism*
Substances
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Intrinsically Disordered Proteins
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Maltose-Binding Proteins
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RNA, Untranslated
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RNA-Binding Protein FUS
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Green Fluorescent Proteins