Direct visualization of the conformational change of FUS/TLS upon binding to promoter-associated non-coding RNA

Chem Commun (Camb). 2020 Aug 11;56(64):9134-9137. doi: 10.1039/d0cc03776a.

Abstract

High-speed AFM revealed the conformational change of fused in sarcoma (FUS) from a compact to an extended structure upon binding of non-coding RNA, which is supposed to allow FUS to bind to CBP/p300 for transcriptional interference. Thus, a mechanistic insight into transcription regulation by FUS and non-coding RNA is provided.

Publication types

  • Video-Audio Media

MeSH terms

  • Green Fluorescent Proteins / chemistry
  • Intrinsically Disordered Proteins / chemistry
  • Intrinsically Disordered Proteins / metabolism
  • Maltose-Binding Proteins / chemistry
  • Microscopy, Atomic Force
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Conformation
  • RNA, Untranslated / metabolism*
  • RNA-Binding Protein FUS / chemistry
  • RNA-Binding Protein FUS / metabolism*

Substances

  • Intrinsically Disordered Proteins
  • Maltose-Binding Proteins
  • RNA, Untranslated
  • RNA-Binding Protein FUS
  • Green Fluorescent Proteins