Flavobacterium restrictum sp. nov., Flavobacterium rhamnosiphilum sp. nov., and Flavobacterium zepuense sp. nov. isolated from glaciers

Abstract

Three strains, designated as LB1R34^T, LB3P52^T and ZT4R6^T, were isolated from glaciers located on the Tibetan Plateau, PR China. The strains were Gram-stain-negative, aerobic, rod-shaped, non-motile and yellow. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that they were related to the members of the genus Flavobacterium. The 16S rRNA gene sequences similarities between the three strains were 92.31-96.93 %. The average nucleotide identity values and digital DNA-DNA hybridization values between these three strains and their closest relatives were 76.80-91.33 % and 21.3-44.2 %, respectively. LB1R34^T, LB3P52^T and ZT4R6^T contained MK-6 as the major menaquinone, summed feature 3 (comprising C_{16 : 1}ω7c and/or C_{16 : 1}ω6c) and iso-C_{15 : 0} as the major fatty acids. Phosphatidylethanolamine was present in their polar lipids profiles. On the basis of the phenotypic characteristics, he results of phylogenetic analysis and genotypic data, three novel species, Flavobacterium restrictum sp. nov. (type strain=LB1 R34^T=CGMCC 1.11493^T=NBRC 113650^T), Flavobacterium rhamnosiphilum sp. nov. (type strain=LB3 P52^T=CGMCC 1.11446^T=NBRC 113776^T) and Flavobacterium zepuense sp. nov. (type strain=ZT4 R6^T=CGMCC 1.11919^T=NBRC 113653^T) are proposed.

Keywords: Flavobacterium restrictum; Flavobacterium rhamnosiphilum; Flavobacterium zepuense.