Data describing expression of formyl peptide receptor 2 in human articular chondrocytes

Alexander Strid Holmertz; Charlotte A Jonsson; Maziar Mohaddes; Christina Lundqvist; Huamei Forsman; Inger Gjertsson; Karin Önnheim

doi:10.1016/j.dib.2020.105866

Data describing expression of formyl peptide receptor 2 in human articular chondrocytes

Data Brief. 2020 Jun 16:31:105866. doi: 10.1016/j.dib.2020.105866. eCollection 2020 Aug.

Authors

Alexander Strid Holmertz¹, Charlotte A Jonsson¹, Maziar Mohaddes², Christina Lundqvist¹, Huamei Forsman¹, Inger Gjertsson¹, Karin Önnheim¹

Affiliations

¹ Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.
² Department of Orthopaedics, Institute of Clinical Sciences, Sahlgrenska Academy, Gothenburg University, Göteborg, Sweden.

Abstract

The formyl peptide receptor 2 (FPR2) belongs to the family of seven-transmembrane G protein-coupled receptors (GPCR) and are expressed by many different cells but mainly studied in immune cells. FPR2 is involved in host defense against bacterial infections and clearance of damaged cells through the oxidative burst and chemotaxis of neutrophils. In addition, FPR2 has also been implicated as an immunomodulator in sterile inflammations, e.g. inflammatory joint diseases. Here we present data regarding FPR2 expression in human articular chondrocytes, isolated from healthy individuals and osteoarthritic patients, on both mRNA and protein level using qPCR and Imagestream flow cytometry. We also present data after receptor stimulation and monitoring of production of nitric oxide, reactive oxygen species, IL-6, IL-8 and MMP-3. The presented data show that human articular chondrocytes from patients with osteoarthritis as well as from healthy individuals express FPR2 both at mRNA and protein level. The biological relevance of FPR2 expression in chondrocytes needs to be further investigated.

Keywords: Articular cartilage; CL, Chemiluminescence; CT, Cycle threshold; Chondrocyte; DMEM, Dulbecco´s modified eagle medium; ECM, Extra cellular matrix; FACS, Fluorescence-activated cell sorting; FBS, Fetal bovine serum; FPR, Formyl peptide receptor; Formyl peptide receptor; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; GPCR, G protein-coupled receptor; HI, Healthy individual; HRP, Horse radish peroxidase; Human; IL-1β, Interleukin 1 beta; KRG, Krebs Ringer phosphate buffer; MMP, Matrix metalloproteinase; NO, Nitric oxide; OA, Osteoarthritis; Osteoarthritis; PBMC, Peripheral blood mononuclear cells; RLU, Relative light units; ROS, Reactive oxygen species; qPCR, Quantitative polymerase chain reaction.